Molecular Biology

Lyrawati D, Trounson A, Cram D (2011) Expression of GFP in the Mitochondrial Compartment Using DQAsome-Mediated Delivery of an Artificial Mini-mitochondrial Genome. Pharm Res. 2011 Nov;28(11):2848-62. Epub 2011 Aug 11.

Raras TYM, Lyrawati D (2011) Cloning and expression of pab gene of Mycobacterium tuberculosis isolated from pulmonary TB patient in Escherichia coli DH5α, Med J Indones. 2011 Nov;20(4):247-54.

Lyrawati D (2009) Mitochondria powerhouse of disease: lecture for postgrad (S3) Fac. Med Brawijaya University.    pdf (low res.)    pdf mt-dis

Published in Jurnal Kedokteran Brawijaya :

Romlah MK., Lyrawati D., Yano K (2015) Nanosecond pulsed electric fields (nsPEFs) induce socs1 and socs3 gene expressions but not socs2 gene expressions on HeLa S3 cells. 2015 Feb; 28(3): 163-169 599-2793-1-PBmartina pdf

Fitria NL., Lyrawati D., Handaru M (2015) Effect of alpha lipoic acid administration on MDA levels and liver histology of male Wistar rats with type 1 diabetes mellitus. 2015 Feb; 28(3): 170-176  608-2795-1-PBLyrawati ALA liver pdf

Putri NF., Lyrawati D., Sarwono I (2015) Effect of alpha lipoic acid on MDA levels and histology of Wistar rats’ kidney type 1 diabetes mellitus. 2015 Feb; 28(3): 177-181  609-2796-1-PBDLyrawati ALA liver pdf

Pramulawati DA., Aurora H., Lyrawati D (2014) The effect of genistein exposure time on zebrafish cardiac formation. 2014 Feb; 28(1): 22-25. LyrawatiDaniorerio411-1165-2-PB genistein cardiac pdf

Lukitasari N., Ratnawati R., Lyrawati D (2014) Polyphenols of Ficus carica Linn inhibit elevation of MCP-1 level of rat with high fat diet. 2014 Feb; 28(1): 1-5.

Rahmahani FN. Indra MR., Lyrawati D. Methanol extract Moringa oleifera leaves affects p53 expression in Rattus norvegicus colon mucosa induced by DMBA. 2013 Aug; 27(4): 207-211.

Khairani F., Ratnawati R., Lyrawati D. Effect of green tea catechins on RORa and C/EBPa ecpressions on preadipocyte primary culture. 2013 Feb; 27(3):131-136.

Mawarti H., Ratnawati R., Lyrawati D (2012) Inhibitory effect of epigallocatechin gallate on insulin resistance in rats with highest diet. 2013 Feb; 27(1): 43-50.

Lyrawati D (2008) Arteriogenesis and angiogenesis in hemorrhagic stroke: refining concept to get the most of neovascularization. 24(1): 44-51   In Press version

Maritha ID., Supranowo, Lyrawati D. (2006) Expression of cytosolic aspartate-specific cysteine protease-3 (Caspase-3) in the liver tissue of Rattus norvegicus (Wistar) following subchronic administration of aflatoxin-B1 (AFB1), Jurnal Kedokteran Brawijaya, 26(3):107-112 ____ pdf 1 2 3 4 5 6

Lyrawati D, Tambunan KB, Handoko HY, Sudoyo H, Marzuki S. (2006)  Polymorphic variants of Adenine Nucleotide Translocator-2 in individuals of normal population and Leber’s Hereditary Optic Neuropathy. 22(1): 30-39  Abstract ANT2 polymorphisms 1 2 3 4 5 6 7 8 9 10 toc OR thesis on

Lyrawati D. (2005) Mitochondrial transfection of murine beta-pancreatic cells and human myoblast cells: a step towards gene therapy. 20(3):1-5 Abstract Mitochondrial Transfection 2


Front cover List of Abbreviation TOC List of Figures

Abstract Declaration Acknowledgements Ammendment


Riset Translasional prepared for Yogya, 2008.

Works writing in progress: GENERATING CYBRIDS CARRYING 16189C>T mtDNA — observation on mitochondrial ultrastructure (2009)

Lyrawati D (2004) Teknik DNA dan genetik (ISBN 979-508-543-3). Teknik Rekombinan DNA dan GenetikTeknik DNA dan Genetik pada Studi Transmisi Penyakit GenetikData berbasis web untuk genetika molekuler dan klinis.

Design and construction of DNA vaccine expressing lectin-like oxidize-LDL receptor-1 (LOX-1) as atherosclerosis vaccine candidate 

Valentina Yurina 1, *, Tri Yudani Mardining Raras 2, Achmad Rudijanto 3, Diana Lyrawati 1, Kusworini Handono 4

1 Department of Pharmacy, 2 Department of Biochemistry and Molecular Biology, 3 Department of Internal Medicine, 4 Department of Pathology Clinic, Medical Faculty, Brawijaya University, Malang, 65145, East Java, Indonesia.

Received: August 4, 2017; accepted: September 22, 2017.

Abstract: Atherosclerosis, a chronic inflammatory disease, is counted as one of the deadliest cardiovascular diseases. Lectin- like oxidized LDL receptor-1 (LOX-1) is the major receptor of the oxidized LDL and the inhibition of LOX-1 can prevent atherosclerosis. The study aimed to predict the epitope of the human LOX-1 protein, to construct the DNA LOX-1 vaccine, and to analyze its expression in the mammalian cell line (HeLa). LOX-1 epitopes were predicted by NetMHC 4.0 Server, NetCTLPan 1.1 Server, PickPocket 1.0 Server, and NetMHC Stab 1.0 Server tools. Plasmids contain LOX-1 epitope were transformed into Escherichia coli DH5α. The correct clone carrying LOX-1 epitope was verified by sequencing analysis. Subsequently, plasmids were transfected into HeLa cells using lipotransfection method. The mRNA expression was analyzed using qRT-PCR and the protein expression was analyzed using Western blot method. From epitopes prediction tools, human LOX-1 contains two epitopes, FLYSPWWCLAAATLGV and WLWEDGSPLMPHLFRV. Both genes and protein level expression analyses result showed that the LOX-1 mRNA expression in the transfected HeLa cells were higher compared to
that in the non-transfected HeLa cells. Our results suggest that pcDNA3.1-LOX-1 is an atherosclerosis DNA vaccine candidate which is recognized in the mammalian cell expression system.

Keywords: atherosclerosis; DNA vaccine; epitope; expression level; HeLa; LOX-1.

*Corresponding author: Valentina Yurina, Pharmacy Department, Medical Faculty, Brawijaya University, Malang, 65145, East Java, Indonesia.
Phone: +62 341 551611. Fax: +62 341 564755. Email:, PDFnya 2017v8p103-11292434valentinayurina


Leave a Reply

Fill in your details below or click an icon to log in: Logo

You are commenting using your account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )


Connecting to %s

%d bloggers like this: